Figure 4.

Effect of MB on NLRC4 or AIM2 inflammasome activation. (A) For NLRC3 inflammasome activation, LPS-primed BMDMs were treated with the indicated dosage of MB in the presence of flagellin (0.5 μg/mL) or Salmonella typhimurium (MOI 3.5) for 1 h. (B) For AIM2 inflammasome activation, macrophages were primed with LPS and treated with MB and dsDNA (1 μg/mL) for 1 h or Listeria monocytogenes (MOI 35) for 3 h. Secretions of caspase-1 (Casp1) and IL-1β were measured by immunoblotting and ELISA. (C) For bactericidal properties of MB, diluted Salmonella or Listeria were cultured in LB or BHI plates with the indicated concentration of MB or antibiotics. G gentamycin (50 μg/mL); P/S, penicillin (100 I.U.), and streptomycin (100 μg/mL). All immunoblot and bacterial growing data shown are representative of at least three independent experiments. Bar graph presents the mean ± SD.