Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Sep 29;7:12440. doi: 10.1038/s41598-017-12124-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Optical projection tomography shows pancreatic beta cell loss in the RIP-DTR mouse. (a,b) Isosurface rendered OPT images of representative splenic lobes of the RIP-DTR mouse at 15 days after sham (a) or diphtheria toxin (b) treatment. Three-dimensional surface renderings are based on anti-insulin staining (red) and intrinsic autofluorescence portraying the anatomy (grey). (c) Graph of the average total pancreatic beta cell volume illustrating severe beta cell loss after diphtheria toxin treatment. (d) Graph showing that beta cell loss in the RIP-DTR pancreas is seen in all three lobes. (e,f) OPT images displaying pseudo-colours yellow (>5 × 10⁶ μm³), red (1–5 × 10⁶ μm³), and white (<1 × 10⁶ μm³) to indicate islets of different sizes in the splenic lobe of control (e) and RIP-DTR (f) pancreas. (g) Graph depicting the overall number of islets in the pancreas of sham or diphtheria toxin treated mice. (h) Graph showing the average cumulative beta cell volume of all islets within their respective size categories. Data presented as mean ± SE with n = 4 animals per group. Statistical significance indicated as ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. Scale bars = 2 mm.