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. 2017 Sep 29;7:12440. doi: 10.1038/s41598-017-12124-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Repeated in vivo confocal imaging details the destruction of RIP-DTR islets over time. (a,b) Consecutively collected photographs (a) and confocal images (b) of engrafted RIP-DTR positive and negative islets in the eye of a diphtheria toxin-resistant control mouse. Yellow bar indicates diphtheria toxin treatment, dashed yellow circles in (a) show examples of individual islets followed over time in (b) using backscattered light to monitor morphological changes. (c) Graph showing the relative islet volume of RIP-DTR positive and negative islets in the anterior chamber of the eye over time following DT treatment. Islet volumes are shown averaged per mouse (grey lines, n = 4–11 islets per eye) and averaged per animal (black lines, n = 4 mice). (d) Relative decrease in islet volume (day 8) of individual RIP-DTR positive islets plotted against their initial starting volume (day 0). Linear regression analysis shows that no size-dependent sensitivity to DT-treatment exists (R² < 0.001, n = 20 islets). Islet size categories as defined for OPT data analysis (“S” = small, “M” = medium, “L” = large, see Fig. 1) are displayed for comparison purposes. Data presented as mean ± SD. Statistical significance indicated as ^***P < 0.001. Confocal images are shown as maximum intensity projections. Scale bars = 50 μm.