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. 2017 Sep 29;7:12401. doi: 10.1038/s41598-017-10861-6

Table 1.

AMOVA results of MSAP based on polymorphic methylation-susceptible loci (MSL).

Period (dpf) Temperature Treatment (°C) d.f. SSD MSD Variance ΦST p
0–15 Constant 15 vs. 17 vs. 19 Among groups 2 238.6 119.360 5.761 0.1626 0
Within groups 45 1335.0 29.670 29.670
Total 47 1574.0 33.480
Acclimation 15 → 19 Among groups 3 449.5 149.8 8.793 0.3611 0
Within groups 59 916.7 15.54 15.54
Total 62 1366 22.04
Acclimation 19 → 15 Among groups 3 462.3 154.1 8.987 0.3531 0
Within groups 59 970.4 16.45 16.45
Total 62 1433 23.11
20–60 Constant 17 vs. 21 Among groups 1 27.6 27.580 −0.032 −0.00115 0.4168736
Within groups 34 957.6 28.170 28.170
Total 35 985.2 28.150

Abbreviations: d.f., degrees of freedom; dpf, days post fertilization; MSD, mean square deviations; MSL, methylation-susceptible loci; SSD, sums of square deviations.