Fig. 6.

Cross-section through the cell body, extensions and varicosities of dopaminergic neurons showing by dual-color dSTORM imaging the distinct distribution of tyrosine hydroxylase (TH) compared to DAT. DAT was visualized by immunolabeling and Alexa647-conjugated secondary antibody. TH was visualized by immunolabeling and CF568-conjugated secondary antibody. a Somatic distribution of DAT. b Somatic distribution of TH. c Merged image of a and b. d–f Enlarged STORM images corresponding to the boxes shown in a–c. g Distribution of DAT in extension with varicosity. h Distribution of TH in extension with varicosity. i Merged image of g and h. j Distribution of DAT in extension. k Distribution of TH in extension. l Merged image of j and k. m Distribution of DAT in varicosity. n Distribution of TH in varicosity. o Merge image of m and n. Scale bars: a–c 5 μm, g–i 2 μm, d–f, j–o 500 nm