Figure 2.

Characterization of purified envelope glycoprotein domain III (EDIII)-specific IgG antibodies raised in mice immunized with different adjuvants. (A) Reactivity of antibodies with the purified antigen following ELISA. (B) Affinity of purified antibodies to the purified EDIII antigen. Values are expressed by percentage of antibodies that remain bound to the solid phase-adsorbed antigen under presence of ammonium thiocyanate in regard to the reaction performed without ammonium thiocyanate. Immunization groups represented by different symbols as indicated in the insert. (C,D) Plaque reduction neutralization test of a DENV2 strain (New Guinea C) carried out with serially diluted serum samples (C) or various amounts of purified EDIII-specific IgG antibodies (D) derived from mice immunized with the different vaccine formulations, as indicated in panel (B). Results are based on two independently performed experiments carried out with duplicate samples. Values represent means ± SD. ***p < 0.001, **p < 0.01, and *p < 0.05, significant differences with regard to mice immunized with non-adjuvanted EDIII (two-way ANOVA with Bonferroni post hoc test). NS: values without statistically significant differences.