Figure 4.

Tregs suppress T595_S597 phosphorylation of DEF6 in responder Tcons. (A) The peptide sequences of DEF6 containing the conserved phosphosites in respective organisms are shown. Ser (S) and Thr (T) with detected phosphorylation in ≥2/3 donors are highlighted in red. Phosphosites of interest (T595 and S597) are additionally highlighted in big letters. (B) log₂ of average ratio of T595_S597 phosphorylated DEF6 peptide in the given comparisons. (C) Representative FTMS spectrum of the indicated DEF6 phosphopeptide (phospho-T595_S597). Precursor areas of the three indicated samples are depicted. Shown is the spectrum from Donor 3 (from one out of three technical replicate mass spectrometry runs) representative of three donors, with following properties: quantified Ion: z = +3, Mono m/z = 744.31616 Da, and MH+ = 2,230.93393 Da. Filled circles are isotope pattern peaks used in calculating the quantification values for the different quantification channels, as opposed to unfilled circles. The bar chart (right) shows quantification of the respective spectrum.