Figure 1.

Higher SIRT7 expression correlates with poor prognosis in osteosarcoma. (A and B) Kaplan-Meier analysis of two probes of SIRT7 (A: 219613_at; B: 233179_at) in survival of osteosarcoma patients (log-rank test; n = 149). The data were obtained from TCGA database. (C) SIRT7 genomic amplification or mRNA expression was analyzed by TCGA database (n = 149). (D) SIRT7 mRNA expression in 58 pairs of osteosarcoma and adjacent normal tissues. The data were obtained from the GEO database. (E) SIRT7 mRNA expression in 32 pairs of osteosarcoma and adjacent normal tissues obtained from Cancer Hospital of China Medical University. (F) The expression of SIRT7 protein were measured by Western blot pairs of osteosarcoma and adjacent normal tissues obtained from Cancer Hospital of China Medical University. (G) The expression of SIRT7 mRNA in osteosarcoma cells were measured by qRT-PCR. (H) The expression of SIRT7 protein in osteosarcoma cells were measured by Western blot. (I) The relative quantitative description of (H). **P < 0.01 (in D, E, G, and I) is based on Student’s t-test. Error bars indicate standard deviation.