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. 2017 Sep 1;7(9):1788–1803.

Figure 2.

Figure 2

SIRT7 induced osteosarcoma cell proliferation in vitro. (A) SIRT7 protein expression was examined by Western blot assay in SIRT7 knockdown SJSA-1 cells. (B) SIRT7 mRNA expression was examined by qRT-PCR assay in SIRT7 knockdown SJSA-1 cells. (C) SIRT7 protein expression was examined by Western blot assay in SIRT7 ectopic expression D-17 cells. (D) SIRT7 mRNA expression was examined by qRT-PCR assay in SIRT7 ectopic expression D-17 cells. (E) Knockdown of SIRT7 inhibited the ability of SJSA-1 cell proliferation measured by MTT. (F) Overexpression of SIRT7 promotes the ability of D-17 cell proliferation measured by MTT. (G) Knockdown of SIRT7 inhibited the ability of SJSA-1 cell proliferation measured by clone formation. (H) Overexpression of SIRT7 promotes the ability of D-17 cell proliferation measured by clone formation. **P < 0.01 (in A, C, E-H) is based on Student’s t-test. Error bars indicate standard deviation.