Figure 3.

SIRT7 induced osteosarcoma cell migration and invasion in vitro. (A) SIRT7 knockdown and its control SJSA-1 cells were subjected to transwell migration (top) and matrigel invasion (bottom) assays. (B) SIRT7 overexpression and its control D-17 cells were subjected to transwell migration (top) and matrigel invasion (bottom) assays. (C) The expression of EMT markers were measured by Western blot in SIRT7 knockdown and its control SJSA-1 cells. (D) The expression of EMT markers were measured by Western blot in SIRT7 overexpression and its control D-17 cells. (E) The expression of EMT markers were measured by IF in SIRT7 knockdown and its control SJSA-1 cells. **P < 0.01 (in A and B) is based on Student’s t-test. Error bars indicate standard deviation.