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. 2017 Sep 1;7(9):1788–1803.

Figure 9.

Figure 9

SIRT7 down regulates H3K18ac expression, and decreases H3K18ac binding to the promoter region of CDC4. (A) The expression of H3K18ac was assayed by Western blot in SJSA-1-shSIRT7 and its control cells. (B) The expression of H3K18ac was assayed by Western blot in D-17-SIRT7 and its control cells. (C) Schematic presentation of three regions relative to the CDC4 transcriptional start site used as primers to test H3K18ac occupied abundance. (D) qChIP was performed to assess H3K18ac occupancy to CDC4 transcriptional start site in SJSA-1-shSIRT7 and its control cells. IgG was used as negative control. (E) qChIP was performed to assess H3K18ac occupancy to CDC4 transcriptional start site in D-17-SIRT7 and its control cells. IgG was used as negative control. **P < 0.01 (in D and E) is based on Student’s t-test. Error bars indicate standard deviation.