Figure 10.

CDC4 is a mediator of SIRT7-induced malignant behaviors in osteosarcoma cells. (A) The silencing of CDC4 was measured by Western blot in SJSA-1-shSIRT-7 cells. (B) The proliferation was measured by MTT in knockdown of CDC4 in SJSA-1-shSIRT7 cells. (C) CDC4 knockdown and its control cells were subjected to transwell migration (top) and matrigel invasion (bottom) assays. (D) Representative phase-contrast images of sphere formation of CDC4 silenced and its control SJSA-1-shSIRT7 cells. (E and F) Quantification of sphere diameter (E) and number (F) of CDC4 silenced and its control SJSA-1-shSIRT7 cells. **,##P < 0.01 (in B, C, E, and F) is based on Student’s t-test. Error bars indicate standard deviation.