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. 2017 Sep 1;7(9):1835–1849.

Figure 2.

Figure 2

MiR-625 overexpression induces cell cycle arrest and inhibits glioma cell growth in vitro. A. CCK-8 assay of proliferation of U87 and U251 glioma cell lines transfected with miR-NC or miR-625. B. Colony-forming assays of U87 and U251 cells transfected with miR-NC or miR-625. C. Representative single or merged images of DAPI- and EDU-stained U87 and U251 cells transfected with miR-NC or miR-625. D. Flow cytometric analysis of cell cycle phase of U251 and U87 cells transfected with miR-NC or miR-625. E. Western blot analysis of Cyclin D1, CDK4, and Cyclin E1 in U87 and U251 cells 48 h after transfection with miR-NC or miR-625. GAPDH served as a loading control. **P < 0.01.