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. 2017 Sep 1;7(9):1835–1849.

Figure 6.

Figure 6

Glioma cell chemosensitivity to TMZ is increased by miR-625, and AKT2 overexpression partially reverses this effect. A. CCK-8 assay of U87 and U251 cells transfected with miR-NC or miR-625 and cultured with the indicated concentrations of TMZ for 48 h. B. CCK-8 assay of U87 and U251 cells transfected with miR-NC, miR-625, or miR-625+AKT2 and cultured with 100 µM TMZ for the indicated times. C. Flow cytometric Annexin V-FITC/PI apoptosis assay of U87 and U251 cells transfected with miR-NC, miR-625, or miR-625+AKT2 and cultured with 100 µM TMZ for 48 h. D. Western blot analysis of O-6-methylguanine-DNA methyltransferase (MGMT) expression in U251 cells stably expressing miR-NC, miR-625, or miR-625+AKT2 and cultured with 100 μM TMZ. GAPDH was used as the loading control. *P < 0.05, **P < 0.01.