Figure 5.

ZWM026 induces different death way of cells and exerts synergistic effect combined with cisplatin. (A, B) Activities of ZWM026 against cleaved-PARP, LC3B and phosphor-PKCα/βII in H1975 and A549 cell lines. Cells were treated with ZWM026 and gefitinib at indicated concentration for 48 hours. The resulting extracts were probed with the indicated antibodies. (C) Efficacy comparisons of ZWM026 and PKC412 against phosphor-EGFR and phosphor-PKCα/βII in constructed NIH-3T3 stably expressed EGFR-L858R/T790M and EGFR-WT. Cells were treated with the compounds for 30 minutes and lysed as described methods. (D) ZWM026 in combination with cisplatin induces the synergistic inhibition of proliferation. We examined the effects of ZWM026 and cisplatin, either alone or in combination, on growth inhibition of H1975 cells by the MTT assay (The CI value was calculated according to the CompuSyn software). The CI > 1 is antagonistic, CI = 1 is additive and CI < 1 is synergistic. *p < 0.05, combination vs. ZWM026, **p < 0.01, combination vs. cisplatin.