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. 2017 Sep 1;7(9):1913–1925.

Figure 3.

Figure 3

PPIs upregulates FOXO1 in SGC-7901 gastric cancer cells. A. TCGA databases show mRNA of FOXO1 have positive correlation with AJCC tumor pathologic in gastric cancer patients. B. SGC-7901 cells were treated with cisplatin (DDP, 10 mg/l), paclitaxel (TAX, 100 nM), and 5-FU (1 mM) combined with scrambled siRNA or FOXO1 siRNA for 24 h. Percentages of cell viability is presented as mean ± S.E.M. (n=5). C. PPIs effect on the mRNA expression of FOXO1 of SGC7901 cells at indicated concentration for 24 h (n=5). D. PPIs effect on the expression of FOXO1 and β-actin of SGC7901 cells at indicated concentration for 24 h (n=3). E. SGC7901 cells were treated with PPIs at indicated concentration for 24 h, and the location of FOXO1 were tested by immunofluorescence. And the nucleus of SGC7901 cells was stained by DAPI (n=3). All photographs were taken at a magnification of ×200. Asterisk indicates a significant difference determined by unpaired two-tailed t test (*** indicates P < 0.001; ** indicates P < 0.01; * indicates P < 0.05).