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. 2017 Sep 15;9(9):4003–4014.

Figure 5.

Figure 5

Re-expression of shRNA-resistant SOX12 rescued the inhibitory effects of shSOX12 on cell proliferation, migration and invasion. SPC-A-1 and A549 cells stably expressing SOX12 shRNA (shSOX12-1) were transfected with shRNA-resistant SOX12 or Vector using Lipofectamine (Invitrogen). (A) SOX12 protein expression was determined at 48 after transfection. (B) Cell proliferation was assessed by CCK-8 assay. (C, D) Cell migration (C) and invasion (D) were evaluated by Transwell assays. shNC: cells stably expressing control shRNA; shSOX12-1, cell clone stably expressing SOX12 shRNA were transfected with Vector; Re-expression, shSOX12-1 cells were transfected with shRNA-resistant SOX12. **P<0.01 and ***P<0.001 VS shNC; ##P<0.01 and ###P<0.001 VS shSOX12-1.