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. 2017 Sep 15;9(9):4003–4014.

Figure 7.

Figure 7

Mechanisms of SOX12 exerts its function. A. mRNA and protein expression of cell growth-related factors (PCNA and Cyclin E) was evaluated by real-time PCR (left panel) and Western blot (right panel), respectively. B. mRNA (left panel) and protein (right panel) expression of apoptotic factors (Bcl-2 and Bax) was assessed. C. mRNA (left panel) and protein (right panel) expression of metastasis (MMP-9) and EMT (Twist1 and E-cadherin) related factors was evaluated. shNC: cells stably expressing control shRNA; shSOX12-1 and shSOX12-2, cell clones stably expressing SOX12 shRNA. **P<0.01 and ***P<0.001 VS shNC. D. SOX12 knockdown affected the binding of SOX12 to Cyclin E promoter and Twist1 promoter in A549 cells as demonstrated by ChIP analysis. **P<0.01 and ***P<0.001 VS shNC/anti-SOX12; ##P<0.01 and ###P<0.001 VS shSOX12-1/IgG.