Table 1.
Primers sequences for real-time PCR
| Primer | Primer sequence | Size (bp) |
|---|---|---|
| SOX12 (NM_006943.3) | F: 5’-AGCACCCGTGTGACTCTTTCC-3’ | 209 |
| R: 5’-AGCAGAACCAAGCCCTGTCTC-3’ | ||
| PCNA (NM_002592.2) | F: 5’-GGTGTTGGAGGCACTCAAGG-3’ | 115 |
| R: 5’-CAGGGTGAGCTGCACCAAAG-3’ | ||
| Cyclin E (NM_001238.2) | F: 5’-TGCGTATGTGACAGATGGAG-3’ | 111 |
| R: 5’-AGCCAGGACACAATAGTCAG-3’ | ||
| Bax (NM_004324.3) | F: 5’-AGCTGAGCGAGTGTCTCAAG-3’ | 248 |
| R: 5’-TGTCCAGCCCATGATGGTTC-3’ | ||
| Bcl-2 (NM_000633.2) | F: 5’-CCACCAGCACCATAGAAG-3’ | 131 |
| R: 5’-GAGCAGGCACAGAGAAAG-3’ | ||
| MMP-9 (NM_004994.2) | F: 5’-AAGGGCGTCGTGGTTCCAACTC-3’ | 210 |
| R: 5’-AGCATTGCCGTCCTGGGTGTAG-3’ | ||
| Twist (NM_000474.3) | F: 5’-AGTCCGCAGTCTTACGAG-3’ | 228 |
| R: 5’-GCTTGCCATCTTGGAGTC-3’ | ||
| E-cadherin (NM_004360.3) | F: 5’-GAGAACGCATTGCCACATACAC-3’ | 164 |
| R: 5’-AAGAGCACCTTCCATGACAGAC-3’ | ||
| GADPH (NM_001256799.1) | F: 5’-CACCCACTCCTCCACCTTTG-3’ | 110 |
| R: 5’-CCACCACCCTGTTGCTGTAG-3’ |