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PPD and metformin corporately trigger ECC’s autophagy. A, B: Ishikawa and RL95-2 cells were treated with PPD (40 uM) and or metformin (20 mM) for 24 h, and then the expression of autophagy-associated proteins Beclin-1, p62 and LC3b was analyzed by western blotting. In addition, the ratio of LC3b II to LC3B was counted by Quantity One analysis soft. Data were representative immune blots of assays.
