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. 2017 Sep 15;9(9):4137–4148.

Figure 4.

Figure 4

Dust mite SIT reduces apoptosis in pulmonary tissue. In vivo, SIT reduced lung apoptosis, and in vitro, IL-25 increased the apoptosis and ROS of 16HBE cells in a time and dose-dependent fashion. A: In the mouse model, the apoptosis of lung tissue was shown with TUNEL staining observed at 10× magnification. B and C: In vitro, apoptosis of 16HBE cells was shown with Annexin V-FITC/PI detection after exposure to various doses of human rIL-25 for different durations (A: control for 12 h; B: 0.1 mg/ml for 12 h; C: 0.1 mg/ml for 24 h; D: control for 24 h; E: 1 mg/ml for 12 h; F: 1 mg/ml for 24 h). D: The levels of ROS in 16HBE cells of the above groups were also tested using flow cytometry. The results are shown as the fluorescence intensity and are representative of 3 independent experiments. The statistics of the results were performed by student t-test. *means P<0.05, **means P<0.01.