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Dust mite SIT upregulates the number of Tregs in the spleen. The spleen from mouse models were homogenized, and the percentage of Tregs (CD4+CD25+Foxp3+) in CD4+T cells was tested by flow cytometry; the results showed (A) the representative and (B) the analysis of 3 independent experiments. The statistics of the results were performed by student t-test. **means P<0.01.
