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. 2017 Sep 15;9(9):4161–4172.

Figure 5.

Figure 5

Astragaloside IV activated autophagy in the ischemic areas of flaps. The autophagy activity in Area II of flap was assessed via Immunofluorescence for LC3 and western blotting for LC3II/I, Beclin1, CTSD, and P62 protein expressions 7 days after surgery. A. Immunofluorescence for the autophagosomes in the cells in Area II of the Control and the AS-IV groups; autophagosomes were labeled via LC3II punctate dots (green), nuclei were labeled with DAPI (blue) (scan bar, 15 μm). B, C. Western blotting was performed for LC3II/I, Beclin1, CTSD and P62 protein expressions in Area II tissue from the Control group and the AS-IV group. Gels were run under the same experimental conditions, and cropped blots are used here. The original images are available in Figure S1D. D-G. Optical density values of LC3II/I, Beclin1, CTSD, and p62 expressions were quantified and analyzed in each group. Significance: *p < 0.05 versus Control group. Data presented as Mean ± SEM, n=6 per group.