Figure 2.

GRHL2 promotes NSCLC cell growth and colony formation. H1299 cells were transfected with indicated siRNAs or not transfected. A. Western blot of GRHL2 to check the knockdown efficiency of siRNAs in the indicated cell lines; β-actin was used as a loading control. B. Growth in low serum. H1299 cells werecultured in 1640 supplemented with 1% serum (low serum) or 10% serum (normal serum). After the indicated time, cells were trypsinized and counted. Data represent the average of three independent experiments. C. Colony formation assay. After the indicated times, H1299 were fixed and stained with crystal violet. Representative Wells were photographed and shown.