Figure 2.
Expression of miR-205-5p regulates chemotherapeutic resistance. The IC50 of 5-Fu was determined using a CCK-8 cell viability assay in Bel cells and multidrug-resistant Bel/Fu cells (A, B). The expression of miR-205-5p was determined in Bel/Fu cells using RT-qPCR and compared with that of Bel cells (C). Expression of miR-205-5p was reduced in Bel cells by 5-Fu treatment, in a dose-dependent manner (D). Bel cells were transfected with hsa-miR-205-5p, a small double-stranded RNA molecule that mimics mature endogenous miR-205-5p, and the transfection efficiency is shown in (E), compared with the non-transfected control (NC). Transfection with the miR-205-5p mimic increased the resistance of Bel cells to 5-Fu, at all doses tested, as determined by a cell viability assay (F). Multidrug-resistant Bel/Fu cells were transfected with a miR-205-5p inhibitor and the transfection efficiency is shown in (G). Transfection with the miR-205-5p inhibitor decreased the resistance of Bel/Fu cells to 5-Fu (H). The data are presented as the mean ± SD of three independent experiments. *p<0.01, §p<0.05.