Figure 4.

Expression of proteins in the PTEN/JNK/ANXA3 pathway. Protein levels were analyzed by Western botting in Bel cells with the indicated doses of 5-Fu treatment, in Bel cells following transfection of a miR-205-5p mimic, and in Bel/Fu cells following transfection of a miR-205-5p inhibitor. All protein band intensities were normalized to the corresponding GAPDH band. NC: non-transfected control; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase; JNK: c-Jun N-terminal kinase; P-JNK: phosphorylated JNK; ANXA3: annexin 3; HIF-1: hypoxia-inducible factor 1; P-gp: P-glycoprotein.