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. 2017 Sep 15;9(9):4308–4316.

Figure 5.

Figure 5

CXCL10 expression is down-regulated by miR-15a-5p in CML cells. A. CXCL10 is predicted as a target gene of miR-15a-5p using TargetScan 7.0. B. Luciferase activity of CXCL10 3’UTR and mutated CXCL10 3’UTR in CML cells with miR-15a-5p mimics transfection. K562 cells were transfected with wide type or mutant type of CXCL10 3’UTR combined with miR-15a-5p mimics and luciferase activity was assayed by dual luciferase assay system. C. qRT-PCR was used to analyze CXCL10 mRNA expression in CML cells. K562 or KU812 cells were transfected with miR-15a-5p mimics and CXCL10 mRNA was assayed by qRT-PCR. D. CXCL10 protein levels in CML cells. K562 or KU812 cells were transfected with miR-15a-5p mimics and CXCL10 protein levels was assayed by Western blotting. E. CXCL10 protein levels in CML cells. K562 or KU812 cells were transfected with miR-15a-5p mimics and CXCL10 protein levels was assayed by ELISA. Error bars are SD. Experiments were conducted in triplicates. **P<0.01.