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Immunofluorescence staining of PAD4 and PAD2 in resting neutrophils. Staining of permeabilized neutrophils with four different anti-PAD4 antibodies as indicated in panel (A) or PAD2 antibody in panel (B). DAPI staining was used to identify nuclear DNA. In parallel samples, recombinant PAD2 or PAD4 were preincubated with antibodies prior to cell staining. Results are representative of three different healthy donors.
