Figure 5.

Molecular modelling of antibiotics’ binding sites onto the glyS T-box:tRNA complex. (A) Schematic representation of the T-box stem I complex with tRNA, illustrating potential interaction sites of neomycin (NMY). The structure of the complex is based on the X-ray structure of T-box riboswitch of Oceanobacillus iheyensis glyQ (blue) in complex with its cognate tRNA (green) from PDB ID 4LCK (25) NMY is shown as CPK model with yellow C, blue N and red O atoms. Close-up views of the four binding sites NMY1 (B), NMY2 (C), NMY3 (D) and NMY4 (E).