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. 2017 Jul 24;45(17):10042–10055. doi: 10.1093/nar/gkx635

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — Spontaneous accumulation of DNA strand breaks in ATM-deficient cells. (A) Alkaline comet assay on TIG1 cells depleted of ATM for the indicated amount of time. The amount of DNA damage, expressed as percentage of DNA in the comet tails, increases in a time-dependent manner (N = 3). (B) Neutral comet assay on TIG1 cells treated as in panel A. The amount of DNA damage, expressed as percentage of DNA in the comet tails, increases in a time-dependent manner (N = 3). Results are expressed as mean ± SD from the indicated number (N) of independent experiments: *P < 0.05; **P < 0.01.