Figure 8.

Complementation of APTX deficiency by pol β and FEN1 for processing of the 5′-AMP-dRP-containing BER intermediate in mitochondrial extracts. Lanes 1–11 and 12–22 are the reaction products observed in mitochondrial extracts from wild-type and APTX^−/− cells, respectively and correspond to time points 1, 3, 5, 10, 15, 20, 25, 30, 40, 50, and 60 min. Illustrations of the single-nucleotide gapped 5′-AMP-dRP-containing DNA substrate and reaction products of pol β, APTX, and FEN1 are as presented in Figure 7A.