Figure 1.

Schematic illustration of the experimental protocol leading to the high-performance liquid chromatography (HPLC)-based measurement of catecholamines [E and norepinephrine (NE)] in rat acute adrenal slice supernatants. The protocol can be divided into four main steps, including (i) the gland slicing using a vibratome, (ii) the challenge of individual slices with drugs of interest, (iii) the calculation of the medulla volume of each challenged slices after a hematoxylin staining, and (iv) the HPLC-based measurement of the amounts of E and NE released by each slice. C, cortex; M, medulla.