Fig. 2.

Identification of the rTspGST. a SDS-PAGE analysis of T. spiralis ML crude antigen (Lane 1), ES antigen (Lane 2) and rTspGST (Lane 3). b Western blot analysis of rTspGST antigenicity. T. spiralis ML crude antigens (Lane 1) and ES antigens (Lane 2) were probed by sera of mice infected with T. spiralis at 42 dpi, but the rTsGST (Lane 3) was not probed by infection sera. The native TspGST protein in ML crude protein (Lane 4) and rTspGST (Lane 6) were probed by anti-rTspGST sera, but the ML ES antigens (Lane 5) were not probed by anti-TspGST sera. T. spiralis ML crude antigens (Lane 7), ES antigens (Lane 8) and rTspGST (Lane 9) were not probed by normal mouse sera. c Western blot analysis indicated that T. spiralis ML crude antigens (Lane 1), ES antigens (Lane 2) and rTspGST (Lane 3) were recognized by sera of mice immunized with ML crude antigens. The ML crude protein (Lane 4) and ES antigens (Lane 5) were recognized by sera of mice immunized with ML ES antigens, but rTspGST (Lane 6) were not recognized by sera of mice immunized with ML ES antigens