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. 2017 Sep 29;8:203. doi: 10.1186/s13287-017-0653-8

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — SHH is expressed in human umbilical cord. a Representative image of immunohistochemistry against SHH in an umbilical cord section. b Imuunohistochemistry shows positive immunoreactivity in cells immersed in the WJ, with hematoxylin staining allowing cell nuclei visualization. c SHH⁺ cells are detected in the WJ by immunofluorescence. d In primary cultures of WJ-MSC, SHH was detected at the cellular surface. e Double immunostaining reveals coexpression of SHH and PTCH1 in WJ-MSC suggesting an autocrine signaling. Scale bars = 0.5 cm (a), 50 μm (b, c), and 20 μm (d, e). A artery, Am amnios, DAPI 4’,6-diamidino-2-phenylindole, PTCH1 Patched1, SHH Sonic hedgehog, V vein, WJ Wharton’s jelly