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. 2017 Sep 29;10:449. doi: 10.1186/s13071-017-2394-z

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — ELISA OD ratios according to hatching order in broods where at least one ELISA-seropositive nestling was found. The black line shows a significant mean decrease in OD ratios (i.e. increase in antibody levels) according to LME model parameter estimate. The grey areas along the y axis depict ELISA classification categories as recommended by the manufacturer. Note that the OD ratio values were jittered along the x-axis. The results show that nestlings that hatched later (i.e. closer to the time of sampling) may have higher antibody levels