Table 1.

Number of ESC-like and AP⁺Nanog⁺ colonies obtained on OSKM/mir-524 co-transduction of HFF-1 cells

Transduction	Colonies in experiment (n)			Total colonies (n)	SD	Reprogramming efficiency (%)
	1	2	3
OSKM only	4	3	6	12	1.53	0.005
OSKM + CD511	6	4	3	12	1.53	0.005
OSKM + mir-524	8	7	12	27*	2.65	0.012*

ESC-like and AP⁺Nanog⁺ colonies in triplicate wells in a 12-well plate were counted under a microscope as described in Methods. Reprogramming efficiency was calculated as the total number of AP⁺Nanog⁺ colonies generated from the total number of transduced HFF-1 cells in three independent experiments. *p < 0.05 compared with OSKM only. AP alkaline phosphatase, ESC embryonic stem cell, SD standard deviation