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. 2017 Sep 29;16:186. doi: 10.1186/s12944-017-0578-3

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Phosphorylation levels of AMPK and ACC protein and thermogenic protein UCP1 in inguinal fat of mice fed SBO-based high-fat diets containing various fractions of BMSO for 14 wk. Representative immunoblot (a). Signals were quantified by image analysis and results expressed as phosphorylated/total protein ratio of AMPK and ACC, as well as ratio of UCP1/GAPDH (b). C, soybean oil-based high-fat diet; BM, soybean oil-based high-fat diet containing BMSO; BMS, soybean oil-based high-fat diet containing saponifiables of BMSO; BMNS, soybean oil-based high-fat diet containing non-saponifiables of BMSO; BMD, soybean oil-based high-fat diet containing bleached BMSO. Results are mean ± SD (n = 10 mice/group) ^a,b Means without a common letter differed (P < 0.05).