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. 2017 Sep 25;8:1646. doi: 10.3389/fpls.2017.01646

Figure 6.

Figure 6

NbAELP/MP binding in vitro does not explain the increased sensitivity to TMV of transgenes with a NbAELP gene knockdown. (A) NbAELP interacts with the TMV MP in vitro in the renatured blot overlay binding assay. The TMV MP was tested for binding to proteins immobilized on the membrane: BG, β-1,3-glucanase (lane 1—1.0 μg, lane 2—0.33 μg); NbAELP (lane 3—1.5 μg, lane 4—0.5 μg); TMV MP (lane 5—0.6 μg). Detection was performed with antibodies against TMV MP. (B) TMV mRNA accumulation in the virus inoculated leaves of proNbAELP-GUS N. benthamiana transgenic plants at the 4th day after inoculation with TMV compared to WT control plants. qRT-PCR analysis of the TMV mRNA content in the leaves of the proNbAELP-GUS N. benthamiana transgenic line 2F of T0 and T1 generations. Error bars represent SE calculated from 3 experimental sets of 10 to 15 plants each. The P-values were used to assess the statistical significance of the differences compared with WT control plants. ***P < 0.001.