Figure 7.

NbAELP is capable of influencing nucleocytoplasmic traffic. (A) Visualization of GFP expression in epidermal cells of leaves co-agroinjected with GFP:NLS^pTα (left) with a projection of several confocal sections superimposed on a bright field image of the same cell (right). Bars = 20 μm. (B) Quantification of GFP:NLS^pTα-directed GFP subcellular localization in the plant leaves after co-agroinjection with 35S-NbAELP or vector control. (C) GFP fluorescent epidermal cell cluster under epifluorescence microscopy at 24 h after agroinjection with 35S-GFP:NLS^pTα alone (left) or in combination with 35S-NbAELP (right). (D) Quantification of the cell clusters shown in (C). At least 500 cell clusters were counted for each experiment. The data represent five independent experiments. The standard error bars and P-value for the statistical significance of the difference between the vector control and 35S-NbAELP-injected leaves are indicated.