Table 2. Name, accession number, primer sequence, probe number, amplicon size, efficiency and R2 of 6 immunologically relevant genes.
| Gene name | Accession number | Primer sequence (5′–3′) | UPL probe number | Amplicon size (bp) | Threshold | Efficiency (%) ± SD | R2 |
|---|---|---|---|---|---|---|---|
| IL-2Rα | XM_004313501 | F-TGAACCTTTGAAGAGAATTTACCA R-CTGAATCCCTGAATGCACTG |
112 | 72 | 0.015 | 98.99 ± 1.25 | 0.998 |
| IL-4 | NM_001280657.1 | F-GCATGGAGCTGCCTGTAGA R-TGCAGAAAGTTTCCTTCTCAGTT |
140 | 69 | 0.012 | 93.92 ± 1.83 | 0.995 |
| IL-10 | AB775207.1 | F-AAGCCCTGTCGGAGATGAT R-CACGTGCTCTTTGATGTTGG |
25 | 86 | 0.012 | 97.25 ± 3.61 | 0.995 |
| IL-12 | XM_004324402.1 | F-CAGAAGGAGCTCTTTTATGACGA R-CCATGTGGTACATCTTCAAGTCC |
98 | 71 | 0.015 | 93.95 ± 3.04 | 0.997 |
| TNF-α | NM_001280615.1 | F-CCAACTGGCTACTCCATCATC R-CGGGCTTGTTACTTGAGGTT |
106 | 76 | 0.012 | 94.44 ± 2.00 | 0.997 |
| IFN-γ | AB022044.2 | F-TTTTCAGCTATGCGTGATTTTG R-TGCATTAAAATATTCCTTTAGGTTTTG |
129 | 94 | 0.010 | 95.78 ± 2.83 | 0.997 |