Table 3. Descriptive statistics and normality evaluation of the reference genes Cq values before and after entering plateau.
Gene | Mean | SD | Min Cq | Max Cq | SW-test p | |
---|---|---|---|---|---|---|
Before plateau | GAPDH | 18.41 | 0.09 | 18.31 | 18.52 | 0.248 |
ACTB | 13.40 | 0.13 | 13.25 | 13.63 | 0.601 | |
18s RNA | 15.84 | 0.56 | 14.79 | 16.63 | 0.989 | |
β2-MG | 15.86 | 0.30 | 15.46 | 16.25 | 0.326 | |
PPIA | 16.82 | 0.18 | 16.66 | 17.09 | 0.095 | |
RPL13A | 13.88 | 0.10 | 13.71 | 14.00 | 0.620 | |
TBP | 21.34 | 0.26 | 20.96 | 21.75 | 0.996 | |
SDHA | 19.79 | 0.26 | 19.41 | 20.17 | 0.963 | |
After plateau | GAPDH | 18.01 | 0.28 | 17.68 | 18.40 | 0.664 |
ACTB | 14.35 | 0.43 | 13.84 | 14.89 | 0.526 | |
18s RNA | 15.79 | 0.29 | 15.32 | 16.10 | 0.616 | |
β2-MG | 16.43 | 0.45 | 15.71 | 16.90 | 0.661 | |
PPIA | 16.96 | 0.30 | 16.38 | 17.26 | 0.089 | |
RPL13A | 13.60 | 0.15 | 13.39 | 13.77 | 0.486 | |
TBP | 21.78 | 0.73 | 20.50 | 22.60 | 0.530 | |
SDHA | 20.11 | 0.32 | 19.67 | 20.57 | 0.987 |
Notes.
- SD
- standard deviation
- Min Cq
- minimum Cq value
- Max Cq
- maximum Cq value
- SW-test p
- p-value of the Shapiro–Wilk test