Hepatic fat content in adult C57BL/6 mice fed a low fat vitamin D replete (LF+), low fat vitamin D deficient (LF−), high fat vitamin D replete (HF+), and high fat vitamin D deficient (HF−) diet. (Panel A) shows the effect of dietary fat within vitamin D replete mice; (Panel B) shows the effect of vitamin D deficiency within low fat diet fed mice. Data shown as mean ± SD, male groups n = 9, female groups n = 7–8, * p < 0.05; (Panel C) shows representative histological microscope images of liver fat content in male mice; (Panel D) shows the equivalent images in female mice. Both high fat and vitamin D deficient diets caused increased lipid accumulation in both sexes, although females had greater lipid accumulation across each comparison.