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. 2017 Sep 11;9(9):1001. doi: 10.3390/nu9091001

Figure 2.

Figure 2

Reactive oxygen species (ROS) generation in HUVECs. Cells were incubated with 0.5% MeOH, tertiary butylhydroperoxide (TBHP), AA, EPA, 5-HETE, or 5-HEPE, for 1 h after treatment with or without 400 μM α-tocopherol for 1 h. ROS were detected using CellROX Green and measured with a flow cytometer, and the ratio of ROS positive cells to total live cell was represented as the percentage of ROS positive cells. Histograms of HUVECs treated with 0.5% MeOH (negative control) and 400μM TBHP (positive control) analyzed by flow cytometry are shown above the graph. Data are expressed as mean ± SD (n = 4). * p < 0.05 compared with 0.5% MeOH; † p < 0.05 compared with 400 μM TBHP; # p < 0.05 and § p < 0.05 compared with 50 μM 5-HETE and 50 μM 5-HEPE, respectively. Statistical analysis was performed with one-way ANOVA followed by Tukey’s test.