Skip to main content
. 2017 Sep 11;9(9):1001. doi: 10.3390/nu9091001

Figure 3.

Figure 3

Figure 3

Effects of antioxidant treatment on nuclear translocation of Nrf2 in HUVECs. Nrf2 translocation was detected by immunostaining (green), and the nuclei were stained with PI (red). HUVECs were pretreated with or without 400μM α-tocopherol for 1 h and followed by incubated for 3 h with each samples: 0.5% MeOH (vehicle) (A); 0.5% MeOH with α-tocopherol (B);10 μM tBHQ (C); 10 μM tBHQ with α-tocopherol (D); 400 μM TBHP (E); 400 μM TBHP with α-tocopherol (F); 50 μM 5-HETE (G); 50 μM 5-HETE with α-tocopherol (H); 50 μM 5-HEPE (I); and 50 μM 5-HEPE with α-tocopherol (J). The intensity of the green fluorescence signal in the nucleus was quantified using Image J (K). Data are expressed as the mean ± SD (n = 8). The intensity of the green fluorescence signal in the nucleus was quantified using Image J (K). Data are expressed as the mean ± SD (n = 8). * p < 0.05 compared with 0.5% MeOH. Statistics analysis was performed with one-way ANOVA followed by Tukey’s test.