Table 1.
The concentrations of 5-HETE, 5-HEPE, and 5-oxo-ETE in the medium and HUVECs.
| Cell (+) | Samples | 5-HETE | 5-HEPE | 5-oxo-ETE |
|---|---|---|---|---|
| Medium (nmol/dish) | MeOH | N.D. | N.D. | N.D. |
| 5-HETE | 54.691 ± 9.554 | 0.066 ± 0.039 | 3.072 ± 0.301 | |
| 5-HEPE | N.D. | 38.886 ± 4.312 | N.D. | |
| 5-oxo-ETE | 0.010 ± 0.009 | N.D. | 0.117 ± 0.010 | |
| Cell (nmol/dish) | MeOH | N.D. | N.D. | N.D. |
| 5-HETE | 0.377 ± 0.062 | 0.002 ± 0.000 | 0.283 ± 0.053 | |
| 5-HEPE | N.D | 0.418 ± 0.064 | N.D | |
| 5-oxo-ETE | 0.005 ± 0.001 | N.D. | 0.002 ± 0.000 |
Cells were incubated with medium containing 0.5% MeOH, 50 μM 5-HETE, 50 μM 5-HEPE, or 5 μM 5-oxo-ETE for 6 h. Harvested cells and medium were deproteinized by acetonitrile containing 0.1% formic acid, and the supernatants were analyzed by LC-MS. Data are expressed as mean ± SD (n = 3). SD, standard deviation; 5-HETE, 5-hydroxyeicosatetraenoic acid; 5-HEPE, 5-hydroxyeicosapentaenoic acid; 5-oxo-ETE, 5-oxo-eicosatetraenoic acid; HUVECs, human umbilical vein endothelial cells; N.D., not detected.