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. 2017 Jul 4;102(10):1718–1726. doi: 10.3324/haematol.2017.171645

Figure 5.

Figure 5

Downregulation of XLOC_109948 lncRNA enhances drug sensitivity in OCI-AML3 cell line. (A) Subcellular localization of XLOC_109948 lncRNA. The RNA level of XLOC_109948 in nuclear and cytoplasmic fraction was evaluated by RT-qPCR after OCI-AML3 cell fractionation. GAPDH was a positive control for cytoplasmic fraction and Snord44 was a positive control for nuclear fraction. (B) Quantification of XLOC_109948 expression in OCI-AML3 cells transiently transfected with two different GapmeRs against XLOC_109948 (a and b), GapmeR Negative Control or water. The RNA expression levels were evaluated by quantitative real-time PCR, normalized to the expressions of TBP and ABL1, and presented as fold change [2−ΔΔCt]±Standard Deviation (SD) (n≥3) relative to cells transfected with the GapmeR Negative Control; ***P<0.0005. (C and D) Apoptosis assay. One day post transfection, cells were treated with (C) Ara-C (10 μM) or (D) ATRA (1 μM), and annexinV/PI staining was performed respectively 24 hours (h) or 48 h later. One representative flow cytometry plot is shown. The histogram represents the average of apoptotic cells (Annexin V+) from four independent experiments. *P<0.05, **P<0.01.