Figure 1.

Notch2 blocking antibody sensitizes hematopoietic stem cell and progenitors (HSPC) egress in response to granulocyte-colony stimulating factor (G-CSF) or/and AMD3100. Mice were given 4 doses of G-CSF in two days, or a single dose of AMD3100, or 4 doses of G-CSF followed by AMD3100 the next day, two days after a single dose (A–C) or 4 doses of Notch2-blocking or control antibody (D–F) (see details of treatment scheme in Online Supplementary Figure S1). Twenty-four hours (h) after the last dose of G-CSF, or 1 h after AMD3100, peripheral blood (PB) was analyzed for white blood cell (WBC) counts (A and D) and the presence of LSK (B and E) and LK (C and F) cells by FACS in PB (n=4–7/group). Results are pooled from 3 independent experiments and presented as mean±Standard Deviation (S.D.). Student t-test *P<0.05, **P<0.01.