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Tight junction integrity. Micrographs depict tight junction proteins ZO-1 (green), claudin-1 (red), and F-actin cytoskeleton (cyan) after 72 h, comparing uninfected control (CTRL−), 1 μM staurosporine control (CTRL+), and G. duodenalis isolate WB6 of MOI 10 and 100. Scale bars indicate 100 μm. Nuclear staining with DAPI is shown in blue. No significant changes in any of the observed proteins due to Giardia-infection are detectable.
