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. 2017 Sep 26;8:1848. doi: 10.3389/fmicb.2017.01848

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Copyright © 2017 Mäki, Salmi, Mikkonen, Kremp and Tiirola.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Comparison of threshold cycles (C_T) of qPCR when amplification of 18S rRNA genes was performed using Euk1A/Euk516R, M13-Euk1A / Euk516R and V8F / 1510r primer pairs from equal quantity of genomic DNA (4 ng). (B) Total wet biomasses, DNA yields, and 18S rRNA gene copy numbers per Power Biofilm extraction. qPCR results are presented as mean values of triplicates with standard errors. ND indicates the sample that was not possible to amplify with the V8F/1510R primer pair. (C) DNA yields per ng of wet biomass. (D) 18S rRNA gene copy numbers per cell and per pg of extracted DNA.