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. 2017 Sep 26;8:1848. doi: 10.3389/fmicb.2017.01848

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Copyright © 2017 Mäki, Salmi, Mikkonen, Kremp and Tiirola.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Relationships of mock community strains based on (A) 18S rRNA gene analyses of DNA extractions and (B) 18S rRNA analyses of RNA extractions, (C) microscopically determined wet biomasses and cell numbers and dry mass and carbon content determinations, and (D) theoretical results based on separately extracted DNA’s of mock strains using Power Biofilm kit. TTR refers to theoretical template relationship, when DNA extracts of strains were isolated and amplified separately counting the results based on individual 18S qPCR results, and pooled DNA refers to a sample containing equal amounts (weight) of DNA of mock strains.